Issue 8.8

Issue 8.10 is now online!

The October issue of Methods is now online!

This double-sized issue contains three Applications articles and two Open Access articles. These five papers are freely available to everyone, no subscription required.

 Phylogenetic TreesThe fields of phylogenetic tree and network inference have advanced independently, with only a few attempts to bridge them. Schliep et al. provide a framework, implemented in R, to transfer information between trees and networks.

 Emon: Studies, surveys and monitoring are often costly, so small investments in preliminary data collection and systematic planning of these activities can help to make best use of resources. To meet recognised needs for accessible tools to plan some aspects of studies, surveys and monitoring, Barry et al. developed the R package emon, which includes routines for study design through power analysis and feature detection.

 Haplostrips: A tool to visualise polymorphisms of a given region of the genome in the form of independently clustered and sorted haplotypes. Haplostrips is a command-line tool written in Python and R, that uses variant call format files as input and generates a heatmap view.

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Monitoring the Distribution and Abundance of Sea Otters

Post provided by Perry Williams

Sea otters (Enhydra lutris) are an apex predator of the nearshore marine ecosystem – the narrow band between terrestrial and oceanic habitat. During the commercial maritime fur trade in the 18th and 19th centuries, sea otters were nearly hunted to extinction across their range in the North Pacific Ocean. By 1911, only a handful of small isolated populations remained.

Sea otters resting in Glacier Bay National Park. © Jamie Womble, NPS. USFWS Permit #14762C-0, NPS Permit #GLBA-2016- SCI-0022.

Sea otters resting in Glacier Bay National Park. © Jamie Womble, NPS. USFWS Permit #14762C-0, NPS Permit #GLBA-2016- SCI-0022.

But sea otter populations have recovered in many areas due to a few changes. The International Fur Seal Treaty in 1911 and the Marine Mammal Protection Act (1972) protected sea otters from most human harvest. Wildlife agencies helped sea otter colonisation by transferring them to unoccupied areas. Eventually, sea otters began to increase in abundance and distribution, and they made their way to Glacier Bay, a tidewater glacier fjord and National Park in southeastern Alaska. Continue reading

Issue 8.8

Issue 8.8 is now online!

The August issue of Methods is now online!

This issue contains two Applications articles and two Open Access articles. These four papers are freely available to everyone, no subscription required.

 Paco: An R package that assesses the phylogenetic congruence, or evolutionary dependence, of two groups of interacting species using both ecological interaction networks and their phylogenetic history.

 Open MEE: Open Meta-analyst for Ecology and Evolution (Open MEE) addresses the need for advanced, easy-to-use software for meta-analysis and meta-regression.It offers a suite of advanced meta-analysis and meta-regression methods for synthesizing continuous and categorical data, including meta-regression with multiple covariates and their interactions, phylogenetic analyses, and simple missing data imputation.

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Assessment of Stream Health with DNA Metabarcoding

Following on from last week’s press release ‘How Clean are Finnish Rivers?’, Vasco Elbrecht et al. have produced a video to explain the methods in ‘Assessing strengths and weaknesses of DNA metabarcoding-based macroinvertebrate identification for routine stream monitoring‘.

In this video, the authors explore the potential of DNA metabarcoding to access stream health using macroinvertebrates. They compared DNA and morphology-based identification of bulk monitoring samples from 18 Finnish stream ecosystems. DNA-based methods show higher taxonomic resolution and similar assessment results as currently used morphology-based methods. Their study shows that the tested DNA-based methods integrate well with current approaches, but further optimisation and validation of DNA metabarcoding methods is encouraged.

This video is based on the article ‘Assessing strengths and weaknesses of DNA metabarcoding-based macroinvertebrate identification for routine stream monitoring‘ by Elbrecht et al.

 

Issue 8.3

Issue 8.3 is now online!

The March issue of Methods is now online!

This issue contains two Applications articles and one Open Access article. These three papers are freely available to everyone, no subscription required.

 Solo: Solo audio recorders are inexpensive, easy to construct and record audible sound continuously for around 40 days. The paper also has a video tutorial explaining how to assemble the required hardware and comes with a companion website with more information.

 The third dimension: A novel design to obtain three-dimensional data on the movements of aquatic organisms at depths of up to 140m. The set-up consists of two synchronised high-speed cameras fixed to two articulated arms and can be used for any underwater applications that require synchronized video recordings of medium- to large-sized animals.

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Issue 7.12

Issue 7.12 is now online!

The final 2016 issue of Methods is now online!

This month’s issue contains four Applications articles and two Open Access articles, all of which are freely available.

– iNEXT: The R package iNEXT (iNterpolation/EXTrapolation) provides simple functions to compute and plot the seamless rarefaction and extrapolation sampling curves for the three most widely used members of the Hill number family (species richness, Shannon diversity and Simpson diversity).

– camtrapR: A new toolbox for flexible and efficient management of data generated in camera trap-based wildlife studies. The package implements a complete workflow for processing camera trapping data.

– rotl: An R package to search and download data from the Open Tree of Life directly in R. It uses common data structures allowing researchers to take advantage of the rich set of tools and methods that are available in R to manipulate, analyse and visualize phylogenies.

– Fluctuating-temperature chamber: A design for economical, programmable fluctuating-temperature chambers based on a relatively small commercially manufactured constant temperature chamber modified with a customized, user-friendly microcontroller.

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Issue 7.11

Issue 7.11 is now online!

The November issue of Methods is now online!

This month’s issue contains four Applications articles and two Open Access articles, all of which are freely available.

– moveHMM: This R package allows ecologists to process GPS tracking data into series of step lengths and turning angles, and to fit a hidden Markov model to these data, allowing, in particular, for the incorporation of environmental covariates.

– BORIS: An open-source and multiplatform standalone program that allows a user-specific coding environment to be set for a computer-based review of previously recorded videos or live observations. Being open to user-specific settings, the program allows a project-based ethogram to be defined that can then be shared with collaborators, or can be imported or modified.

– inbreedR: An R package that provides functions to measure variance in inbreeding – through the strength of correlation in heterozygosity across marker loci – based on microsatellite and SNP markers with associated P-values and confidence intervals. Within the framework of Heterozygosity–fitness correlation theory, inbreedR also estimates the impact of inbreeding on marker heterozygosity and fitness.

– Terrestrial Precipitation Analysis: This package is comprised of the Precipitation Trends (P-Trend), Precipitation Attributes (P-Att) and Precipitation Manipulation (P-Man) tools. Combined, these web tools allow researchers to easily calculate fundamental precipitation statistics for past, present and projected future precipitation regimes for any terrestrial location in the world.

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Automatic Camera Monitoring: A Window into the Daily Life of Pollinators

Post provided by Ronny Steen

Image from the Canon PowerShot camera with CHDK script ‘Motion Detect Plus’. The thistle flower being visited by ♀ honeybee Apis mellifera L.

Image from the Canon PowerShot camera with CHDK script ‘Motion Detect Plus’. The thistle flower being visited by ♀ honeybee Apis mellifera L.

Pollinators have fascinated ecologists for decades, and they have traditionally been monitored by on-site human observations. This can be a time-consuming enterprise and – more importantly – species identification and recordings of behaviour have to be registered at the time of observation. This has two complications:

  1. While writing notes, or recording them electronically, the observer cannot continue focusing on the animal or behaviour in question.
  2. Such data then have to be transcribed, with the risk of making transcription errors.

Bringing Monitoring into the 21st Century

Although on-site human observations have predominated, today’s widespread availability of digital monitoring equipment has enabled unique data on flower visitors to be collected. In my research, I have used a time-efficient automated procedure for monitoring flower-visiting animals – namely foraging bumblebees visiting focal white clovers and honeybees visiting thistles.

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Issue 7.7

Issue 7.7 is now online!

The July issue of Methods is now online!

This month’s issue contains two Applications articles and two Open Access articles, all of which are freely available.

– MO-Phylogenetics: A software tool to infer phylogenetic trees optimising two reconstruction criteria simultaneously and integrating a framework for multi-objective optimisation with two phylogenetic software packages.

– PHYLOMETRICS: An efficient algorithm to construct the null distributions (by generating phylogenies under a trait state-dependent speciation and extinction model) and a pipeline for estimating the false-positive rate and the statistical power of tests on phylogenetic metrics..

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Canopy Camera Trapping: Heightening Our Knowledge of Arboreal Mammals

Post provided by FARAH CARRASCO-RUEDA and TREMAINE GREGORY

We wanted to test whether arboreal mammals were using natural canopy bridges – connections between tree branches over a clearing – to travel over a natural gas pipeline in the Peruvian Amazon. The challenge was figuring out how to monitor branches 100 feet up in the tree tops. In this case, the clearing was a 30-foot-wide pipeline path, and we expected arboreal mammals – like monkeys, squirrels and porcupines – to prefer crossing on the branches rather than on the ground. The ground is an unfamiliar and often dangerous place for an animal that’s spent its life way up in the canopy.

The path captured by the camera.

The yellow arrow shows the path captured by the camera trap.

In fact, we wondered if without branches, would arboreal mammals cross at all? How could we find out if animals were using the branches? There were 13 canopy bridges and finding a person to sit and wait all day (and night) under each of them for animals to cross wasn’t an option. With our goal of a year’s worth of monitoring, we had a conundrum. We needed a more efficient way to gather the data and concluded that camera traps – motion sensitive cameras – could be an excellent way to monitor the bridges continuously and remotely.

But, we discovered that no one had ever really used camera traps in the high canopy before. How were we going to get them all the way up there? If we were able to get up to the canopy, how could we make sure they were taking photos of the correct points where animals would potentially cross? Continue reading