Assessment of Stream Health with DNA Metabarcoding

Following on from last week’s press release ‘How Clean are Finnish Rivers?’, Vasco Elbrecht et al. have produced a video to explain the methods in ‘Assessing strengths and weaknesses of DNA metabarcoding-based macroinvertebrate identification for routine stream monitoring‘.

In this video, the authors explore the potential of DNA metabarcoding to access stream health using macroinvertebrates. They compared DNA and morphology-based identification of bulk monitoring samples from 18 Finnish stream ecosystems. DNA-based methods show higher taxonomic resolution and similar assessment results as currently used morphology-based methods. Their study shows that the tested DNA-based methods integrate well with current approaches, but further optimisation and validation of DNA metabarcoding methods is encouraged.

This video is based on the article ‘Assessing strengths and weaknesses of DNA metabarcoding-based macroinvertebrate identification for routine stream monitoring‘ by Elbrecht et al.

 

New Associate Editors

Today we are welcoming two new people to the Methods in Ecology and Evolution Associate Editor Board. Pierre Durand is joining us from the University of the Witwatersrand (South Africa) and Andrew Mahon joins from Central Michigan University (USA). You can find out more about Pierre and Andrew below.

Pierre Durand

Pierre Durand

“My research is broadly focussed on the evolution of complexity. Many of my projects are related to the evolutionary ecology of programmed cell death (PCD) in unicellular organisms; how PCD impacts microbial communities; and how the philosophy of levels of selection informs our understanding of PCD evolution. I have also examined other aspects of complexity evolution such as the origin of life and group formation in unicellular chlorophytes in response to predation. The model organisms I typically use are phytoplankton. With specific reference to submissions to Methods in Ecology and Evolution, I have used a range of methods in my research, including general cell and molecular biology tools, biochemical assays, microscopy, flow cytometry, bioinformatics and computational algorithms.”

The most current projects in Pierre’s laboratory concern: programmed cell death evolution and complexity in microbial communities; changes in phytoplankton abundance and diversity in harmful algal blooms, led by PhD candidate Andrew Ndhlovu (“A red tide forming dinoflagellate Prorocentrum triestinum: identification, phylogeny and impacts on St Helena Bay, South Africa” in review in Phycologia); and the genomics of the four-celled chlorophyte Tetrabaena socialis, led by PhD candidate Jonathan Featherson.

Andrew Mahon

Andrew Mahon

“I’m a molecular ecologist who uses genetic and genomic tools to ask questions ranging from surveillance and monitoring to biodiversity and phylogeography.  My work includes development of novel molecular detection tools and metabarcoding applications for aquatic invasive species.  I’m also interested in applying molecular tools to ask questions related to the evolution and biodiversity of benthic marine invertebrates in Antarctica.”

Andrew has recently been published in the journal Research Ideas and Outcomes (‘DNAqua-Net: Developing new genetic tools for bioassessment and monitoring of aquatic ecosystems in Europe‘) and in Environmental Science and Technology (‘Influence of Stream Bottom Substrate on Retention and Transport of Vertebrate Environmental DNA‘). He also has a manuscript in press with Ecology and Evolution (‘Geographic structure in the Southern Ocean circumpolar brittle star Ophionotus victoriae (Ophiuridae) revealed from mtDNA and single-nucleotide polymorphism data‘).

We are thrilled to welcome Pierre and Andrew to the Associate Editor Board and we look forward to working with them over the coming years.